METHOD FOR DETECTING, DIFFERENTIATING, AND QUANTIFYING T CELL POPULATIONS BY MEANS OF REVERSE TRANSCRIPTION QUANTITATIVE REAL-TIME PCR (RT-QPCR) TECHNOLOGY

摘要

The invention relates to a method for detecting, differentiating, and quantifying T cell populations, comprising the following steps: a) bringing a first aliquot of a bodily fluid of an individual in contact with at least one antigen, wherein the bodily fluid contains antigen-presenting cells (APCs) and T cells, b) incubating the first aliquot with the at least one antigen over a certain time period, c) detecting and differentiating the T cell populations by detecting at least one first marker of the APCs induced by the T cells of a certain T cell population in the first aliquot and in a second aliquot of the bodily fluid of the individual, which was not incubated with the at least one antigen, by means of reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), and d) detecting and quantifying the T cell populations by determining the ratio of the detected markers of the APCs of the first aliquot to the second aliquot. The invention further relates to a kit for carrying out the method.