发明名称 |
CELL FOR PREPARING COMPETENT CELL, METHOD FOR PREPARING COMPETENT CELL AND BACTERIAL STRAIN OF ESCHERICHIA COLI |
摘要 |
The invention provides a cell for preparing competent cells, wherein the cell is capable of spontaneously accumulating self-producing trehalose therein and the cell is used for the preparation of competent cells. The invention also provides a method for preparing competent cells, including: culturing the cell for preparing the competent cell mentioned previously to obtain a cell suspension; placing the cell suspension into an ice bath; centrifuging the cell suspension to obtain a cell precipitate; mixing a transform reagent with the cell precipitate; and obtaining competent cells suspension. |
申请公布号 |
US2012149090(A1) |
申请公布日期 |
2012.06.14 |
申请号 |
US20100979079 |
申请日期 |
2010.12.27 |
申请人 |
WANG JIA-HUNG;TSAI MENG-YIN;HUNG TING-TING;TENG KELLY |
发明人 |
WANG JIA-HUNG;TSAI MENG-YIN;HUNG TING-TING;TENG KELLY |
分类号 |
C12N1/21;C12N1/15;C12N1/19;C12N1/20 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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