METHOD FOR DEVELOPING GENE SPECIFIC MOLECULAR MARKERS

摘要

PURPOSE: A method for developing a frogeye leaf spot resistant gene-specific molecular marker is provided to distinguish different species at a genomic level. CONSTITUTION: A method for developing a molecular marker comprises: a step of isolating genomic DNA with different reactivities to frogeye leaf spot; a step of searching for a gene which is similar to a sequence of the MYB transcription regulatory gene of Glycine m from the DNA base sequence database of Medicago truncatula; a step of searching for a promoter site from the database; a step of designing a PCR primer for amplifying a specific site of the promoter; a step of amplifying the promoter site using a primer and genomic DNA; and a step of confirming a pattern.