发明名称 |
Direct quantification of ribosome inactivating protein |
摘要 |
A method for detecting ribosome inactivating protein (RIP) activity in a sample is provided that involves contacting a sample that contains an RIP with an inventive substrate that is depurinated to form product. The product is hybridized to a template and cleaved by an apurinic/apyrimidinic endonuclease such that releasing the blocked 3′ end only in a sample that contains RIP activity. The 5′ end of the product serves as a primer for extension by a polymerase reaction. The newly synthesized strand complementary to the template is detected by RT-PCR processes. A kit is provided suitable for field or laboratory use. |
申请公布号 |
US8188257(B1) |
申请公布日期 |
2012.05.29 |
申请号 |
US20100824323 |
申请日期 |
2010.06.28 |
申请人 |
DORSEY RUSSELL M.;THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE ARMY |
发明人 |
DORSEY RUSSELL M. |
分类号 |
C07H21/04;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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