A 3' -BASED SEQUENCING APPROACH FOR MICROARRAY MANUFACTURE

摘要

Disclosed is a method of designing a nucleic acid microarray comprising: isolating RNA from a tissue sample; sequencing transcripts from the isolated RNA using an oligo dT primer to derive extreme 3' oligonucleotide sequences of the RNA; using the extreme 3' oligonucleotide sequences to design probes for the microarray; and producing a microarray possessing the probes directed to an extreme 3' end of the RNA to account for variable extreme 3' ends or alternative polyadenylation in the tissue sample.