| 摘要 |
The invention relates to a method for simultaneously detecting at least one specific DNA target molecule in a plurality of samples by means of nucleic acid amplification, comprising the following steps: a) providing at least two samples comprising DNA, b) bringing the samples from step a) in contact with a forward primer and a reverse primer comprising a 3' and a 5' portion, wherein the forward and reverse primers in the 3' portion contain a nucleotide sequence that is complementary to the amplifying DNA target molecule, and the forward or reverse primer in the 5' portion comprises an oligonucleotide having an artificial DNA sequence that is associated with the individual samples, c) amplifying the samples from step b), d) bringing the amplified samples from step c) in contact with a solid substrate on which oligonucleotides are immobilized at a preselected site, said oligonucleotides comprising at the 3' ends thereof the artificial DNA sequence associated with the individual samples, and e) ascertaining the bond of the amplification products from step c) at the oligonucleotides immobilized on the solid substrate.
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