发明名称 |
FLOCCULATION WITH DIVALENT SALT AND PHOSPHATE |
摘要 |
A method of flocculating a bacterial cell, producing a protein of interest, from a fermentation broth, comprising a) diluting the fermentation broth up to 1000% (w/w) with water; b) adding a divalent salt to a concentration in the fermentation broth of more than 10 milli moles per liter diluted fermentation broth; c) adjusting the phosphate concentration to a concentration in the fermentation broth of more than 10 milli moles per liter diluted fermentation broth; d) adjusting the pH of the diluted fermentation broth to a pH within the range of 6.1-10.5; and e) removing the bacterial cells, whereby a protein solution with a turbidity of less than 100 NTU is obtained. |
申请公布号 |
US2012122183(A1) |
申请公布日期 |
2012.05.17 |
申请号 |
US201013382500 |
申请日期 |
2010.06.30 |
申请人 |
PEDERSEN JAKOB RAUHE;PIND PETER FRODE;NOVOZYMES A/S |
发明人 |
PEDERSEN JAKOB RAUHE;PIND PETER FRODE |
分类号 |
C12N9/20;C12N9/00;C12N9/42;C12N9/44;C12N9/52;C12N9/54 |
主分类号 |
C12N9/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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