A RECOMBINANT VIRUS BEASYBAC AND BACULOVIRUS EXPRESSION VECTOR SYSTEM USING THE SAME

摘要

PURPOSE: A baculovirus expression vector system using a recombinant virus bEasyBac is provided to enhance separation efficiency of the recombinant virus and to enable high-throughput of the recombinant virus. CONSTITUTION: A baculovirus expression vector system simultaneously produces a large amount of foreign gene-introduced recombinant virus and is bEasyBac(deposit number:KCTC 11499BP). The foreign gene is EGFP gene and is inserted in to a transfer vector pDualBac(5,964 bp). The expression system has Kanamycin resistant gene and mini F replicon. The expression system contains a lethal gene isolated from Bacillus amyloquefaciens and ORF 3005 promoter.