发明名称 |
Method of producing a population of post-mitotic cells of the neutrophil lineage |
摘要 |
A method is provided for producing a population of post-mitotic cells of the neutrophil lineage, which method comprises the ex vivo steps of: (a) providing a population of cells comprising neutrophil progenitor cells; and (b) culturing the population of cells in an animal cell culture medium comprising (i) one or more early acting cytokines and (ii) one or more cytokines that differentiate said progenitor cells into a neutrophil specific lineage, under conditions of low oxidative stress, the culture medium being agitated when the cells are at a cell density at which oxygen transfer via the surface of the culture medium is insufficient for growth of the progenitor cells and the progeny thereof under static conditions, to produce a population of post-mitotic cells of the neutrophil lineage. The resulting population of cells can be used to increase the number of neutrophils in a patient. |
申请公布号 |
US8173427(B2) |
申请公布日期 |
2012.05.08 |
申请号 |
US20070375098 |
申请日期 |
2007.07.23 |
申请人 |
NIELSEN LARS K.;PALFREYMAN EMMA L.;TIMMINS NICHOLAS E.;THE UNIVERSITY OF QUEENSLAND |
发明人 |
NIELSEN LARS K.;PALFREYMAN EMMA L.;TIMMINS NICHOLAS E. |
分类号 |
C12N5/00;C12N5/02;C12N5/071;C12N5/0787 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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