发明名称 |
Methods and Compositions for Concentrating Secreted Recombinant Protein |
摘要 |
Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein. |
申请公布号 |
US2012107877(A1) |
申请公布日期 |
2012.05.03 |
申请号 |
US201213346258 |
申请日期 |
2012.01.09 |
申请人 |
TARON CHRISTOPHER H.;COLUSSI PAUL A.;NEW ENGLAND BIOLABS, INC. |
发明人 |
TARON CHRISTOPHER H.;COLUSSI PAUL A. |
分类号 |
C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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