发明名称 |
METHOD FOR THE RECOMBINANT PRODUCTION OF PEPTIDIC ANTIVIRAL FUSION INHIBITORS, AND ACETYLATION OF GB41 FRAGMENTS |
摘要 |
A process for the production of an antifusogenic peptide of a length of about 10 to 50 amino acids in a prokaryotic host cell, characterized in that, under such conditions that inclusion bodies of said non-fusion antifusogenic peptide or said fusion peptide are formed, a) in said host cell there is expressed a nucleic acid encoding said antifusogenic peptide as a non-fusion peptide or encoding a fusion peptide of a length of about 14 to 70 amino acids consisting of said antifusogenic peptide N-terminally linked to a further peptide of a length of about 4 to 30 amino acids; b) said host cell is cultivated; c) said inclusion bodies are recovered and solubilized; d) in the case of said fusion peptide said antifusogenic peptide is cleaved off from said further peptide; and e) said antifusogenic peptide is isolated.
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申请公布号 |
CA2450548(C) |
申请公布日期 |
2012.05.01 |
申请号 |
CA20022450548 |
申请日期 |
2002.05.27 |
申请人 |
F. HOFFMANN-LA ROCHE AG |
发明人 |
HOESS, EVA;MEIER, THOMAS;PESTLIN, GABRIELE;POPP, FRIEDRICH;REICHERT, KLAUS;SCHMUCK, RAINER;SCHNEIDINGER, BERND;SEIDEL, CHRISTOPH;TISCHER, WILHELM |
分类号 |
C12N15/09;C12P21/02;A61K38/00;A61P31/12;A61P31/18;C07K14/155;C07K14/16;C12N1/21;C12N15/62 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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