Preparations and compositions are provided herein that comprise an isolated lactoferrin and a nutritional mixture and that does not include an inorganic source of a biologically effective amount of iron. In particular embodiments, preparations and compositions are provided herein that comprise an isolated lactoferrin and a nutritional mixture comprising at least folic acid and Vitamin B6 in amounts sufficient to supplement daily dietary requirements of an adult, including a pregnant subject, an infant, or a child..