METHOD FOR DETERMINING A BIOLOGICAL PATHWAY ACTIVITY

摘要

Method for determining a level of activity of a biological pathway from a biological sample of a patient suffering from a pathology, said method comprising the steps of: measuring the level of expression of at least three genes from a group of individuals, control individuals or patients, for which the biological pathway is inactive, said at least three genes being associated to said biological pathway, for establishing a negative reference, and measuring level of expression of said at least three genes from a group of individuals, control individuals or patients, for which the biological pathway is active, for establishing a positive reference, and measuring the level of expression of said at least three genes of said patient in the sample, and comparing the level of expression of said at least three genes in the sample of said patient with the level of expression of said at least three genes of the negative reference and determining a value C- which corresponds to a correlation level between the level of expression of said at least three genes of said negative reference and the level of expression of said at least three genes of said patient, and comparing the level of expression of said at least three genes in the sample of said patient with the level of expression of said at least three genes of the positive reference and determining a value C+ which corresponds to a correlation level between the level of expression of said at least three genes of said positive reference and the level of expression of said at least three genes of said patient, and establishing a ratio C+/C- which gives a correlation score, wherein if the correlation score is lower to 1 that is indicative of the non activity of the biological pathway, and if the correlation score is upper or equal to 1 that is indicative of the activity of the biological pathway.