MICROBIOLOGICAL METHOD OF PRODUCING 1,2-DEHYDROGENATED DERIVATIVES OF ö4-3-KETO-STEROIDS OF ANDROSTANE FAMILY IN AQUEUS ORGANIC MEDIA

摘要

FIELD: chemistry. ^ SUBSTANCE: disclosed is a method of producing 1,2-dehydrogenated derivatives of ö4-3-ketosteroids of the androstane family. A 1,2-dehydrogenation reaction is carried out using cells of a microorganism having high 3-ketosteroid-1-dehydrogenase activity, in a medium containing 8-40 vol. % water miscible aprotic solvent. ^ EFFECT: invention enables transformation of ö4-3-ketosteroids and ensures high selectivity of formation of 1,2-dehydrogenated ö4-3-ketosteroids at high rates of the process. ^ 4 cl, 1 tbl, 5 ex