发明名称 |
A porous membrane device that promotes the differentiation of monocytes into dendritic cells |
摘要 |
Dendritic cells (DCs) for research and clinical applications are typically derived from purified blood monocytes that are cultured in a cocktail of cytokines for a week or more. Because it has been suggested that these cytokine-derived DCs may be deficient in some important immunological functions and might not accurately represent antigen-presenting cell (APC) populations found under physiologic conditions, there is a need for methods that allow the generation of DCs in a more physiologically relevant manner. The present invention comprises a simple and reliable technique for generating large numbers of highly purified DCs, based on a single migration of blood monocytes through endothelial cells that are cultured in, for example, a Transwell® device. The resultant APCs, harvested from the lower Transwell® chamber, resemble other -generated DC populations in their expression of major histocompatibility (MHC) and costimulatory molecules, ability to phagocytose foreign antigens, and capacity to trigger antigen-specific T cell responses. |
申请公布号 |
AU2006330951(B2) |
申请公布日期 |
2012.04.05 |
申请号 |
AU20060330951 |
申请日期 |
2006.12.21 |
申请人 |
SANOFI PASTEUR VAXDESIGN CORPORATION |
发明人 |
PARKHILL, ROBERT;HIGBEE, RUSSELL;MOE, DAVID;DRAKE III, DONALD;SANCHEZ-SCHMITZ, GUZMAN;WARREN, WILLIAM L.;FAHLENKAMP, HEATHER;LI, CONAN |
分类号 |
C12N5/0784;G01N33/00 |
主分类号 |
C12N5/0784 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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