| 摘要 |
Provided is a method for the isolation of long fragment RNA from a fixed tissue sample comprising the following: a) heating the fixed tissue sample in an extraction solution to a temperature in the range of about 44 to about 62° C for a time period of 3 hours or more, where in the extract ion solution comprises a chelator at a concentration of about 0. 1 mM to about 20 mM, and proteinase K; and b) removing DNA contamination by performing at least a first and a second extract ion with a solution comprising phenol and chloroform, wherein the second extraction further comprises a chaotropic agent, to produce an RNA-enriched solution; and c) isolating said RNA from said RNA-enriched solution.
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