| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for efficient mass-synthesis of CMP-deaminoneuraminic acid (CMP-KDN) and a method for purifying the product. <P>SOLUTION: There is provided the method for producing CMP-KDN by using CMP-N-acetylneuraminic acid synthetase (CMP-NeuAc synthetase) as an enzyme in the method for enzymatically producing the CMP-deaminoneuraminic acid (CMP-KDN) from cytidine 5'-triphosphate (5'-CTP) and deaminoneuraminic acid (KDN). There is further provided the CMP-KDN purification method comprising the following steps 1 to 4. (Step 1) a step to add a bivalent cation to a solution containing CMP-KDN and precipitate coexisting phosphoric acid, pyrophosphoric acid and nucleotides; (step 2) a step to convert the nucleotide to a nucleoside by an alkaline phosphatase reaction; (step 3) a step to precipitate CMP-KDN by an organic solvent; and (step 4) a step to recover the CMP-KDN. <P>COPYRIGHT: (C)2012,JPO&INPIT |
