| 摘要 |
A microscope apparatus comprises: a distribution measurement apparatus with respect to an observation region that comprises a fluorescent material that is activated when irradiated with an activating light, and that emits fluorescence when irradiated with an exciting light of a wavelength different from the activating light, obtains a fluorescent picture image through irradiation with the exciting light, and measures a fluorescent intensity distribution; an irradiation intensity setting apparatus which sets irradiation intensities of the activating light for respective portions of the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus which obtains a plurality of the fluorescent picture images by repeating an operation wherein the observation region is irradiated with the activating light at the set irradiation intensities, and repeating an operation wherein a fluorescent picture image is obtained by irradiating with the exciting light after the activating light irradiation, generating a sample picture image. |
