MODIFIED CELL CO-EXPRESSING BLIMP1 AND REPORTER MOLECULE, AND METHOD FOR USING THE SAME

摘要

PROBLEM TO BE SOLVED: To provide a genetically modified cell or a non-human organism comprising such cells, comprising a modified genetic material producing a polypeptide co-expressed with a reporter molecule on its expression.

SOLUTION: Here, this polypeptide is associated with the terminal differentiation of a hematopoietic cell. The gene of the genetic substance is Blimp allele or a part, fragment or functional form of the same. Further, the identification of the reporter molecule in B cell lineage cells indicates that such cells are committed to differentiation to ASC (antibody secreting cell) or have differentiated to the ASC. Alternatively, the activity of the reporter molecule in T cell lineage cells indicates that these cells are activated. Thus, the presence of the Blimp in a lymphocyte indicates that the cell is terminally differentiated or is committed to terminal differentiation. Exemplary T cells include CD4+T cells and CD8+T cells, and exemplary B cells are the ASC.

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