UNCOUPLING OF DNA INSERT PROPAGATION AND EXPRESSION OF PROTEIN FOR PHAGE DISPLAY METHOD

摘要

PROBLEM TO BE SOLVED: To provide a method for providing an advance in phage display technology by permitting the uncoupling of the propagation of phages containing inserted sequences encoding heterologous polypeptides from the expression of the polypeptides.

SOLUTION: There are provided phage constructs and methods for using the same to permit phage coat protein expression, and thus the propagation of phages, in the absence of display of the heterologous polypeptides, which can be expressed as a fusion with the coat protein in a regulated manner.

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