| 摘要 |
A method for making suspended microarray readings from a single sample more reliably accurate. It can be applied to any assay system that uses discrete particles coupled with an assay. Most of these are fluidic systems that read the assay result using flow cytometry. However, other methods such as the distribution of tiny assay devices coupled with miniature transponders, where the sampling is of the environment, can also make use of this method. This invention combines a reference set of signal levels on particles with separately identified assays of one sample. By elimination of outliers, averaging, taking ratios of averages, and then taking ratios of assay signal levels against the reference set this method makes possible highly reliable diagnostics. When used standalone, the method uses different signal intensities to better calibrate an instrument. This method compensates for multiple sources of errors that can occur in this type of assay system. |
