METHOD FOR PRODUCING MATURE VWF FROM VWF PRO-PEPTIDE

摘要

Disclosed is a method for producing mature von Willebrand Factor (VWF) from von Willebrand Factor pro-peptide comprising the steps of: (a) Immobilizing VWF pro-peptide on an ion exchange resin; (b) Incubating the immobilized VWF pro-peptide with furin comprising activity of at least 0.2 Units furin/Unit VWF antigen (Ag) to obtain immobilized mature VWF; and (c) Isolating at least 90% mature VWF from the ion exchange resin by elution.