TANNIC ACID-DEGRADABLE LACCASE 3 OF CRYPHONECTRIA PARASITICA AND USAGE THEREOF

摘要

PURPOSE: A novel laccase protein and a method for preparing the same are provided to be used in pulp, food, and fiber industries. CONSTITUTION: A laccase 3 which decomposes tannic acid is isolated from Cryphonectria parasitica. The laccase 3 has an amino acid of sequence number 7. A recombinant expression vector contains a region encoding laccase 3. The region is a region encoding mature laccase 3 and is regulated by inducible promoter. The inducible promoter is GAL1(galactose kinase) promoter. The recombinant expression vector is pYEGLAC3 or pYEGALLAC3. A yest host cell transformed by the recombinant expression vector is Saccharomyces sp.