发明名称 |
VECTORS FOR EXPRESSION OF HML-2 POLYPEPTIDES |
摘要 |
<p>A nucleic acid vector comprising: (i) a promoter; (ii) a sequence encoding an HML-2 polypeptide operably linked to said promoter; and (iii) a selectable marker. Preferred vectors comprise: (I) an eukaryotic promoter; (ii) a sequence encoding an HML-2 polypeptide downstream of and operably linked to said promoter, (iii) a prokaryotic selectable marker; (iv) a prokaryotic origin of replication; and (v) an eukaryotic transcription terminator downstream of and operably linked to said sequence encoding an HML-2 polypeptide. Vectors of the invention are particularly useful for expression of HML-2 polypeptides either in vitro (e.g. for later purification). Or in vivo (e.g. for nucleic acid immunization). They are well suited to nucleic acid immunization against prostrate tumors. A preferred HML-2 is PCAV, which is located in chromosome 22 at 20.428 megabases (22q11.2).
A virus-like particle (VLP) comprising HML-2 gag polypeptides and its use in immunization and treatment or diagnosis of prostate tumor.</p> |
申请公布号 |
EP1532161(B1) |
申请公布日期 |
2012.02.15 |
申请号 |
EP20030741966 |
申请日期 |
2003.06.13 |
申请人 |
NOVARTIS VACCINES AND DIAGNOSTICS, INC. |
发明人 |
HARDY, STEPHEN, F.;DONNELLY, JOHN, J., III;ZUR MEGEDE, JAN, T. |
分类号 |
C07H21/04;C12N15/09;A61K31/7088;A61K35/76;A61K39/00;A61K39/21;A61K48/00;A61P3/10;A61P25/00;A61P35/00;C12N7/00;C12N7/01;C12N15/48;C12N15/63;C12N15/74;G01N33/569 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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