发明名称 |
METHODS FOR CULTURE AND PRODUCTION OF SINGLE CELL POPULATIONS OF HUMAN EMBRYONIC STEM CELLS |
摘要 |
We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency. |
申请公布号 |
US2012034618(A1) |
申请公布日期 |
2012.02.09 |
申请号 |
US201113164639 |
申请日期 |
2011.06.20 |
申请人 |
TERSKIKH ALEXEY;BAJPAI RUCHI;SANFORD-BURNHAM MEDICAL RESEARCH INSTITUTE |
发明人 |
TERSKIKH ALEXEY;BAJPAI RUCHI |
分类号 |
C12N5/0735;C12Q1/02;G01N21/64 |
主分类号 |
C12N5/0735 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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