| 摘要 |
The invention relates to the field of fluorescent proteins and their versatile applications in the field of molecular biology. More specifically, the invention relates to methods to produce genetically engineered fluorescent proteins for high efficiency FRET (Förster Resonance Energy Transfer). In particular, such methods comprise fusing a donor and acceptor fluorescent protein to a low-affinity helper interaction module. Moreover, pairs of such genetically engineered fluorescent proteins are provided as well as uses thereof in FRET-based applications.
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