| 摘要 |
The invention belongs to the technical field of genetic engineering of agricultural microorganisms. Specifically, the invention relates to producing and using gene cluster for thuringiensin synthesis from Bacillus thuringiensis. The gene cluster includes 11 genes of thuA, thuB, thuC, thuD, thuE, thuF, thuG, thu1, thu2, thu3 and thu4. These genes have the nucleotide sequence as shown in SEQ ID NO: 1. And the thuringiensin biosynthetase encoded by these genes have the amino acid sequences as shown in SEQ ID NO: 2-12. The genes according to the invention and the proteins encoded by those genes can be used as new nucleosides lead compound of pesticide library and provide new target for developing insecticides. In practical use, a series of new, efficient and low toxic insecticide can be obtained by structure modification.
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