| 摘要 |
<p>An isotachophoresis method for preconcentrating and isolating a plurality n of charged analytes (A i , with i = 1 to n) contained in a sample is disclosed, wherein each one of the analytes A i has an effective electrophoretic mobility µ Ai obeying the fully ordered relationship µ A1 > µ A2 > etc. > µ An , comprising the step of preparing a mixture of said sample and a number n-1 of spacer compounds (S k , with k = 1 to n-1) wherein each one of said spacer compounds (S k ) has an effective electrophoretic mobility µ Sk obeying the fully ordered relationship µ Ak > µ Sk > µ Ak+1 . An axial electric field is applied along the longitudinal axis of a separation channel, thereby causing a preconcentration and separation of the analytes and spacers forming respective focused spacer zones and focused analyte zones that flow along the longitudinal axis. Each one of the spacer compounds (S k ) has an initial concentration ( c 0 , Sk ) selected in such manner as to substantially correspond, at its preconcentrated concentration ( c Sk ) in the respective focused spacer zone, to a volume of the main separation channel enclosed between an associated pair of adjacent extraction channels (E k ) and (E k+1 ). Through this spatial displacement of analytes A i by spacers S k , the analytes can be selectively extracted into the extraction channels E i .</p> |
