A PREPARATION METHOD OF SOLUBILITY RECOMBINANT PROTEIN BY USE OF GLUTATHIONE SYNTHETASE AS A FUSION EXPRESSION PARTNER

摘要

PURPOSE: A method for preparing recombinant proteins using glutathione synthetase(GshB) is provided to maintain structural stability and to improve solubility and folding ability. CONSTITUTION: A method for preparing recombinant proteins comprises: a step of preparing an expression vector containing glutathione lsynthetase(GshB) gene and a gene of foreign protein; a step of introducing the expression vector to a host cell to obtain a transformant; a step of culturing the transformant under the stress condition to collect recombinant proteins. The host cells are E.coli.