NATURAL KILLER CELL RECEPTOR GENOTYPING METHOD USING MULTIPLE GENE AMPLIFICATION

摘要

The present invention relates to a method for the genotyping of human natural killer cell immunoglobulin-like receptors (referred to hereinbelow as "KIR"). The method comprises: a multiple gene simultaneous amplification step (1st PCR) in which a plurality of mutually different KIR template genes are simultaneously amplified by using a primer comprising sequences common to all KIR types; a gene amplification step (2nd PCR) using a primer comprising sequences specific to each KIR type; and a step of KIR genotyping of a person being tested, by using bead array or the like to analyse the KIR genes amplified in this way.