Glucanases, nucleic acids encoding them and methods for making and using them

摘要

The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.