HIGH-THROUGHPUT OPTICAL TECHNIQUE FOR DETECTING BIOMOLECULAR INTERACTIONS

摘要

An instrument system for detecting biochemical interactions on a biosensor, said instrument system comprising:
(a) a biosensor comprising:
(i) a two-dimensional grating;
(ii) a substrate that supports the two-dimensional grating; wherein the refractive index of the two-dimensional grating is greater than the refractive index of the substrate; and
(iii) one or more specific binding substances immobilized on the surface of the two-dimensional grating opposite of the substrate; wherein, when the biosensor is illuminated a resonant grating effect is produced on a reflected or transmitted radiation spectrum of incident light by generation of a leaky mode in the grating; and wherein the depth and period of the two-dimensional grating are less than the wavelength of the resonant grating effect;
(b) a light source;
(c) a first optical fiber coupled to the light source, wherein the first optical fiber illuminates the biosensor;
(d) a second optical fiber that detects a signal reflected or transmitted from the surface of the biosensor; and
(e) a spectrometer for determining spectra of the reflected or transmitted signal from the biosensor.