HIGHLY SENSITIVE METHOD FOR DETECTION OF A TARGET NUCLEIC ACID IN A SAMPLE

摘要

The present invention relates, in a first aspect, to a highly sensitive method to detect a target nucleic acid in a sample, comprising a step a) of amplification of the target nucleic acid sequence in presence of at least of one primer and one probe, wherein the primer melting temperature (Tm) is at least 5°C above the melting temperature (Tm) of the probe and said amplification is carried out at a temperature above the Tm of said probe in order to allow hybridization of the primer to the target nucleic acid sequence essentially in the absence of hybridization of said probe; and a step b) of amplification and detection by RT-PCR of the signal produced by hybridization of said probe to the target nucleic acid sequence.