发明名称 |
PROCESS FOR PRODUCING RECOMBINANT PROTEIN USING NOVEL FUSION PARTNER |
摘要 |
The present invention provides a method of producing polypeptide utilizing a fusion protein of A-B type in the following formula (I), by culturing transformed microorganism comprising DNA sequence encoding the desirable polypeptide; A-B (I). In the above formula (I), A is a fusion partner of 25 or more amino acid residues where aspartic and glutamic acid residues are incorporated to have a net negative charge of 30% or more, and B is the target protein to be produced. The target protein can be isolated from the fusion protein by employing enzymatic cleavage site etc. at the carboxyl-terminus of the fusion partner. |
申请公布号 |
US2011318779(A1) |
申请公布日期 |
2011.12.29 |
申请号 |
US20100735242 |
申请日期 |
2010.06.24 |
申请人 |
SHIN HANG-CHEOL;JANG SEUNG-HWAN;KO EUN-HYE;KIM HYO-JIN;PARK YEAN-HEE;KIM MYUNG-HWAN;YOON KI-HOON;SONG HYANG-DO;HYUN HYE-RAN;NUTREX TECHNOLOGY CO., LTD. |
发明人 |
SHIN HANG-CHEOL;JANG SEUNG-HWAN;KO EUN-HYE;KIM HYO-JIN;PARK YEAN-HEE;KIM MYUNG-HWAN;YOON KI-HOON;SONG HYANG-DO;HYUN HYE-RAN |
分类号 |
C12P21/06;C07K19/00;C12N1/21;C12N15/63;C12P21/02 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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