METHOD FOR Escherichia coli BACTERIA IDENTIFICATION BY DETECTING THEIR FRAGMENTS BY ATOMIC-FORCE MICROSCOPY

摘要

FIELD: medicine. ^ SUBSTANCE: method involves specific immobilisation of fragments of the specified bacteria from a solution on an affine surface consisting of a protein G layer coated with a layer of antibodies specific to the identified bacterial cells. Two-stage washing of a sample from a non-specifically bound material implies washing in a buffer pH 8.5-9.5 and washing in deionised water. The atomic-force microscopy is used as a visualisation tool of the bound bacterial fragments and enables direct count of a bound analyte amount per a surface unit. ^ EFFECT: offered invention allows identifying the minute cell concentration in the analysed solution. ^ 1 dwg