发明名称 |
Method for Generating a Recombinant Clonal Cell Line and Novel Reagents for Use in the Method |
摘要 |
A method for generating a recombinant clonal cell line expressing a target cell surface receptor at a specific level of expression from a cell population comprising cells transfected with a plasmid encoding the cDNA sequence of the target receptor and expressing the target cell surface receptor, the method comprising (c) incubating the cell population with a receptor specific fluorescent ligand (d) selecting single cells from step (c) expressing the target cell surface receptor by monitoring the specific binding of the fluorescent ligand using flow cytometry; and novel fluorescent ligands. |
申请公布号 |
US2011300116(A1) |
申请公布日期 |
2011.12.08 |
申请号 |
US200813133366 |
申请日期 |
2008.09.29 |
申请人 |
HILL STEPHEN JOHN;KELLAM BARRIE;MIDDLETON RICHARD JOHN;CELLAURA TECHNOLOGIES LTD |
发明人 |
HILL STEPHEN JOHN;KELLAM BARRIE;MIDDLETON RICHARD JOHN |
分类号 |
A61K35/12;A61K35/00;A61P43/00;C07D223/16;C07D487/14;C07F5/02;G01N21/64 |
主分类号 |
A61K35/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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