发明名称 |
High fidelity restriction endonucleases |
摘要 |
Inter alia, a composition characterized in that it comprises: a restriction endonuclease enzyme having at least one artificially introduced mutation and an overall fidelity index (FI) improvement factor of at least 2, the restriction endonuclease being capable of cleaving a substrate with at least a similar cleavage activity to that of the restriction endonuclease absent the artificially introduced mutation, in a predetermined buffer, wherein the artificially introduced mutation is the product of at least one of a targeted mutation, saturation mutagenesis, or a mutation introduced through a PCR amplification procedure, and wherein the restriction endonuclease absent the artificially introduced mutation is Sbfl and the artificially introduced mutation is K251A is disclosed. |
申请公布号 |
EP2392653(A1) |
申请公布日期 |
2011.12.07 |
申请号 |
EP20110075107 |
申请日期 |
2008.07.14 |
申请人 |
NEW ENGLAND BIOLABS, INC. |
发明人 |
ZHU, ZHENYU;BLANCHARD, AINE;XU, SHUANG-YONG;GUAN, SHENGXI;WEI, HUA;ZHANG, PENGHUA;SUN, DAPENG;CHAN, SIU-HONG |
分类号 |
C12N9/16;C12N9/22;C12N15/10 |
主分类号 |
C12N9/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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