MICROORGANISM FOR QUANTIFYING HOMOCYSTEINE AND USE THEREOF

摘要

PURPOSE: A microorganism for quantitation of homocysteine and methionine is provided to ensure accurate and quick quantitation of homocysteine in several samples. CONSTITUTION: A method for quantiation of homocysteine in a sample using auxotrophic mutant E.coli comprises: a step of culturing mutant E.coli strain of homocysteine and methionine auxotrophy in the sample to measure the content of homocysteine and methionine in the sample; a step of culturing mutant E.coli strain of methionine auxotrophy in the sample to measure the methionine content in the sample; and a step of subtracting the methionine content from the content of homocysteine and methionine in the sample. The E.coli strain of methionine auxotrophy is E.coli Met5-(KCCM11070P). The content is measured by expression level of luciferase or fluorescent protein. The quantitation is used for diagnosing cardiac disease, pregnancy complications, osteoporosis, or Alzheimer's disease.