| 摘要 |
Embodiments of the invention are related to a polypeptide comprising the amino acid sequence of a human IgE-Fc C&egr;3-C&egr;4, wherein said C&egr;3-C&egr;4 starts at amino acid 328 and ends at amino acid 547 of said IgE-Fc, and wherein C 328 is A and K 367 is C. Other embodiments concern a second polypeptide comprising the amino acid sequence of a human Fc&egr;RIα extracellular region, wherein said extracellular region starts at amino acid 1 and ends at amino acid 176 of said Fc&egr;RIα. Still other embodiments are related to a method of identifying a compound that inhibits the binding of an IgE-Fc to a Fc&egr;RIα, said method comprising: contacting the polypeptide, wherein said IgE-Fc C&egr;3-C&egr;4 sequence is labeled with a fluorophore, and the second polypeptide, with a test compound; and determining whether binding of said polypeptide to said second polypeptide is decreased in the presence of said test compound. |
