METHOD TO INCREASE CLASS I PRESENTATION OF EXOGENOUS ANTIGEN BY HUMAN DENDRITIC CELL

摘要

PROBLEM TO BE SOLVED: To provide methods and compositions for use of human dendritic cells to activate T cells for immunotherapeutic responses against primary and metastatic cancer.

SOLUTION: In one embodiment, human dendritic cells exposed to a tumor associated antigen, or an antigenic fragment thereof in combination with bacillus Calmette-Guerin (BCG), are administered to a cancer patient to activate a predominantly CD8+T cell response in vivo. In an alternate embodiment, human dendritic cells are exposed to a tumor associated antigen or a specific antigenic peptide in combination with BCG in vitro and incubated or cultured with primed or unprimed T cells to activate a predominantly CD8+T cell response in vitro. The activated T cells are then administered to a cancer patient. Antigen in combination with BCG is processed by dendritic cells through the MHC-CLASS I compartment which provides for a predominantly CD8+T cell response.

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