发明名称 |
OLIGONUCLEOTIDE LIGATION, BARCODING AND METHODS AND COMPOSITIONS FOR IMPROVING DATA QUALITY AND THROUGHPUT USING MASSIVELY PARALLEL SEQUENCING |
摘要 |
Described herein is a buffer concentration for highly efficient ligation of two oligonucleotides. The embodiments herein have led to the development of an optimized ligation step used in the sample preparation for sequencing reactions. Further, embodiments herein describe a high-throughput method for sequencing using barcodes or the purpose of multiplexing several samples simultaneously and novel methods for making targeted DNA libraries for re-sequencing on massively parallel next-generation sequencing platforms and for alternatives to gel-purification for recovering the desired templates from small RNA libraries for next generation sequencing. |
申请公布号 |
WO2011140510(A2) |
申请公布日期 |
2011.11.10 |
申请号 |
WO2011US35633 |
申请日期 |
2011.05.06 |
申请人 |
BIOO SCIENTIFIC CORPORATION;TOLOUE, MASOUD, M.;GOLDRICK, MARIANNA |
发明人 |
TOLOUE, MASOUD, M.;GOLDRICK, MARIANNA |
分类号 |
C12Q1/68;C12N15/10;C12N15/11 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|