Methods for obtaining immortalized antibody secreting cells

摘要

Disclosed is a method for immortalising cells that secrete antibodies of one or more specific isotypes. The method comprises selecting the population of cells that expresses antibodies from one or more biological samples in an antigen-independent manner and on the basis of the expression of at least a cell surface marker; stimulating said population of selected cells, eliminating the stimulating agent from the cell culture; selecting the population of stimulated cells that expresses antibodies of one or more isotypes from said cell culture and exposing said population of selected and stimulated cells to the immortalizing agent in cell culture conditions.