| 摘要 |
Disclosed is a simple and low-cost method for strengthening and improving the nuclease degradation resistance of single-chain or two-chain nucleic acid fragments containing a base sequence of functional nucleic acids. Specifically, hairpin DNA is attached to at least one of the ends of the single-chain nucleic acid fragment or two-chain nucleic acid fragment. This hairpin DNA is formed from a nucleic acid region (A) formed from 2 - 5 random nucleotides, a nucleic acid region (B) formed from a base sequence of gna or gnna (wherein each n is, independently, any of g, t, a, or c, a base analog or a modified base), and a nucleic acid region (C) formed from a complementary base sequence for nucleic acid region (A) such that these regions are attached sequentially from the 5' end side toward the 3' end side. |
