METHOD FOR THE IDENTIFICATION OF T CELL EPITOPES

摘要

A novel method to identify relevant T-cell epitopes recognized by CD8+ or CD4+ T lymphocytes is described. The method is based on the use of mRNA fragments synthesized from cDNA encoding portions of a polypeptide of interest. mRNA fragments are introduced into antigen-presenting cells to deduce an epitope's localization in a polypeptide of interest, such as a protein antigen.