| 摘要 |
There is provided a method and reagents for detecting S. enterica subsp. IIIa and/or IIIb in a sample, the method comprising: (a) contacting the sample with a pair of forward and reverse oligonucleotide primers, wherein said forward and reverse primers hybridise to target nucleic acid sequences located within the lacZ gene of S. enterica subsp. III, or the complement thereof; (b) extending said forward and reverse primers to generate an amplification product; and (c) detecting the amplification product. There is also provided a method a reagents for detecting S. enterica subsp. I in a sample, the method comprising: (a) contacting the sample with a pair of forward and reverse oligonucleotide primers, wherein said forward and reverse primers hybridise to target nucleic acid sequences located within the hilA gene of S. enterica subsp. I, or the complement thereof; (b) extending said forward and reverse primers to generate an amplification product; and (c) detecting the amplification product.
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