Method and computer-program product for detecting and quantifying protein spots

摘要

The present inventions is directed to a method and computer program product for detecting and quantifying protein spots, including: generating an average gel image by taking a pixel-by-pixel average of the intensities of a plurality of aligned gel images; detecting spots on the average gel image using pinnacle detection; and quantifying spots on individual gels using the maximum intensity within fixed neighborhoods surrounding pinnacle locations found in the average gel image.