METHOD FOR CLONING COGNATE ANTIBODIES

摘要

Disclosed is a method of producing a library of cognate pairs comprising linked variable region encoding sequences. The method comprising: a) providing a lymphocyte-comprising cell fraction from a rodent donor; b) obtaining a population of isolated single cells by distributing cells from said cell fraction individually into a plurality of vessels, wherein at least a subpopulation of the cells are enriched in and express CD43 and CD138 antigen or MHCII and B220 antigen; and c) amplifying and effecting linkage of the variable region encoding sequences contained in said population of isolated single cells by amplifying in a multiplex molecular amplification procedure, nucleotide sequences of interest using a template derived from an isolated single cell or a population of isogenic cells; and effecting linkage of the amplified nucleotide sequences of interest. Also disclosed is a library of vectors obtained by the method.