摘要 |
An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems. |
申请人 |
CALIFORNIA INSTITUTE OF TECHNOLOGY;TRUONG, THAI, V.;CHOI, JOHN, M.;FRASER, SCOTT, E.;SUPATTO, WILLY;KOOS, DAVID, S. |
发明人 |
TRUONG, THAI, V.;CHOI, JOHN, M.;FRASER, SCOTT, E.;SUPATTO, WILLY;KOOS, DAVID, S. |