ANTITUBERCULOSIS COMPOSITIONS OF BYTTNERIA SPECIES

摘要

In the present study, active fractions exhibiting abti-tuberculous activity from the plant Byttneria herbecea (family - Sterculiaceae) is discussed. Methanol extracts of the aerial part of Byttneria herbecea exhibited activity against MtbGS in primary screening of biosynthetic assay. Subsequent fractionation was carried out in organic solvents. Out of all these, two fractions (fraction D and K) are exhibiting inhibitory activity against M.tuberculosis glutamine synthetase activity. These fractions D and K are inhibiting 74% and 44% respectively at 7.5mg/ml concentrations. The determined IC50 value of the fraction K is found to be 4.5 mg/ml from the dose response curve against the purified Mycobacterium tuberculosis Glutamine Synthetase. The major advantage of the present study is that the mode of action of the compound is known, which increases the potential of Byttneria herbecea for becoming an anti-tubercular herbal drug. The IC50 value for M. bovis BCG is 1.56 µg/ml. It was observed that there was no significant effect on proliferation of HL-60 cell line at 10 X MIC levels of the hits. This data indicated that these compounds could be initially considered to be safe.