Fluorescence detecting device and fluorescence detecting method

摘要

The present invention relates to a fluorescence detecting device and a fluorescence detecting method which is a fluorescence intensity calculating method of obtaining respective fluorescence intensities based on detected values of fluorescence that is generated by irradiating a plurality of labeled samples with a laser beam, the samples being labeled by a plurality of fluorochromes. The method comprises the steps of time-modulating an intensity of the laser beam in time at a given frequency, irradiating the labeled samples with the laser beam, and receiving the fluorescence generated by the labeled samples by a plurality of detection sensors of different light receiving wavelength bands, to thereby acquire the detected values including phase information from the respective detection sensors; producing the correction conversion matrix by setting matrix elements of the correction conversion matrix; and obtaining, with a set of the detected values including the phase information acquired from the respective detection sensors as a vector, the fluorescence intensities of the fluorescence that is generated by the labeled samples by allowing an inverse matrix produced from the correction conversion matrix to act on the vector. The fluorescence detecting method is characterized in that each of the set matrix elements of the correction conversion matrix has a portion of a transfer function incorporating a fluorescence relaxation time of each fluorescence of the labeled samples that are irradiated with the laser beam when it is assumed that each fluorescence of the labeled samples is a relaxation response of a first order lag system.